The 3M™ Pine Nut Protein ELISA Kit is intended for the detection of pine nut proteins in clean-in-place water (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.
The 3M Pine Nut Protein ELISA Kit utilizes a sandwich ELISA. The pine nut proteins present in the sample react with the anti-pine nut antibody, which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-pine nut antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound pine nut protein. Following a second washing step, the enzyme bound to the immunosorbent is detected by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The color development from this enzymatic reaction varies directly with the concentration of pine nut protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of pine nut protein in the test sample. The quantity of pine nut protein in the test sample can be extrapolated from the standard curve, constructed from standards of known concentration, and adjusted to consider the sample dilution.